Part:BBa_K1861302:Design

eYFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 4
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 671
  

Design Notes

This is a component of BBa_K1861030.
In order to clone eYFP into BBa_K1861030, we had to add the restriction sites for XhoI and SalI to BBa_E3020. We used the following primers to achieve this:

5' XhoI primer - TCT GCT CGA GGC AACTAG CGG CAT G

3' SalI primer - CTA CGT CGA CTT ATG CGGTAC CAG AAC CTT TG
The restriction sites are in italic.
As the start codon ATG lies before the XhoI site, the resulting protein will begin with the amino acids MLE.
The start codon ATG of BBa_E2030 was removed by the XhoI primer, as the last G of the restriction site corresponds to the G of the start codon of BBa_E2030.

Source

We used the sequence of BBa_E2030.

References

This part is too simple to warrant references.